Gut check: How healthy microbes in infancy reduce diabetes risk
Jennifer Hill, assistant professor of molecular, cellular and developmental biology, works in a new germ-free lab designed for the study of the infant gut microbiome. Credit: Patrick Campbell/91福利社
Exposure to antibiotics during a key developmental window in infancy can stunt the growth of insulin-producing cells in the pancreas and may boost risk of diabetes later in life, new research in mice suggests.
The study, , also pinpoints specific microorganisms that may help those critical cells proliferate in early life.
The findings are the latest to shine a light on the importance of the human infant microbiome鈥攖he constellation of bacteria and fungi living on and in us during our first few years. The research could lead to new approaches for addressing a host of metabolic diseases.
鈥淲e hope our study provides more awareness for how important the infant microbiome actually is for shaping development,鈥 said first author Jennifer Hill, assistant professor in molecular, cellular and developmental biology at CU鈥檚 BioFrontiers Institute. 鈥淭his work also provides important new evidence that microbe-based approaches could someday be used to not only prevent but also reverse diabetes.鈥

Jennifer Hill in her office at the BioFrontiers Institute. Credit: Patrick Campbell
Something in the environment
More than 2 million U.S. adults live with Type 1 diabetes, an incurable disease in which the pancreas fails to make insulin (the hormone that turns glucose into energy) and the blood fills with sugar.
The disease typically emerges in childhood, and genetics play a strong role. But scientists have found that, while identical twins share DNA that predisposes them to Type 1 diabetes, only one twin usually gets the disease.
鈥淭his tells you that there鈥檚 something about their environmental experiences that is changing their susceptibility,鈥 said Hill.
For years, she has looked to microbes for answers.
Previous studies show that children who are breastfed or born vaginally, which can both promote a healthy infant microbiome, are less likely to develop Type 1 diabetes than others. Some research also shows that giving babies antibiotics early can inadvertently kill good bugs with bad and boost diabetes risk.
The lingering questions: What microbes are these infants missing out on?
鈥淥ur study identifies a critical window in early life when specific microbes are necessary to promote pancreatic cell development,鈥 said Hill.
A key window of opportunity
She explained that human babies are born with a small amount of pancreatic 鈥渂eta cells,鈥 the only cells in the body that produce insulin.
But some time in a baby鈥檚 first year, a once-in-a-lifetime surge in beta cell growth occurs.
鈥淚f, for whatever reason, we don鈥檛 undergo this event of expansion and proliferation, that can be a cause of diabetes,鈥 Hill said.
She conducted the current study as a postdoctoral researcher at the University of Utah with senior author June Round, a professor of pathology.
They found that when they gave broad-spectrum antibiotics to mice during a specific window (the human equivalent of about 7 to 12 months of life), the mice developed fewer insulin producing cells, higher blood sugar levels, lower insulin levels and generally worse metabolic function in adulthood.
鈥淭his, to me, was shocking and a bit scary,鈥 said Round. 鈥淚t showed how important the microbiota is during this very short early period of development.鈥
Lessons in baby poop
In other experiments, the scientists gave specific microbes to mice, and found that several they increased their production of beta cells and boosted insulin levels in the blood.
The most powerful was a fungus called Candida dubliniensis.

June Round holds a sample in the lab at University of Utah Health. Photo: Courtesy of University of Utah Health
The team used fecal samples from The Environmental Determinants of Diabetes in the Young (TEDDY) study to make what Hill calls 鈥減oop slushies鈥 and fed them to the mice.
When the researchers inoculated newborn mice with poop from healthy infants between 7 to 12 months in age, their beta cells began to grow. Poop from infants of other ages did not do the same.
Notably, Candida dublineinsis was abundant in human babies only during this time period.
鈥淭his suggests that humans also have a narrow window of colonization by these beta cell promoting microbes,鈥 said Hill.
When male mice that were genetically predisposed to Type 1 diabetes were colonized with the fungus in infancy, they developed diabetes less than 15% of the time. Males that didn鈥檛 receive the fungus got diabetes 90% of the time.
Even more promising, when researchers gave the fungus to adult mice whose insulin-producing cells had been killed off, those cells regenerated.
Too early for treatments
Hill stresses that she is not 鈥渁nti-antibiotics.鈥
But she does imagine a day when doctors could give microbe-based drugs or supplements alongside antibiotics to replace the metabolism-supporting bugs they inadvertently kill.
Poop slushies (fecal microbiota transplants) have already been used experimentally to try to improve metabolic profiles of people with Type 2 diabetes, which can also damage pancreatic beta cells.
But such approaches can come with real risk, since many microbes that are beneficial in childhood can cause harm in adults. Instead, she hopes that scientists can someday harness the specific mechanisms the microbes use to develop novel treatments for healing a damaged pancreas鈥攔eversing diabetes.
She recently helped establish a state-of-the-art 鈥済erm-free鈥 facility for studying the infant microbiome at 91福利社. There, animals can be bred and raised entirely without microbes, and by re-introducing them one by one scientists can learn they work.
鈥淗istorically we have interpreted germs as something we want to avoid, but we probably have way more beneficial microbes than pathogens,鈥 she said. 鈥淏y harnessing their power, we can do a lot to benefit human health.鈥
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